Toon star Roger is worried that his wife Jessica is playing pattycake with someone else, so the studio hires detective Eddie Valiant to snoop on her. But the stakes are quickly raised when Marvin Acme is found dead and Roger is the prime suspect.

Bob Hoskinsas Eddie Valiant
Christopher Lloydas Judge Doom
Joanna Cassidyas Dolores
Charles Fleischeras Roger Rabbit / Benny the Cab / Greasy / Psycho (voice)
Stubby Kayeas Marvin Acme
Alan Tilvernas R.K. Maroon
Richard LeParmentieras Lt. Santino
Lou Hirschas Baby Herman (voice)
Betsy Brantleyas Jessica's Performance Model
Dean Cundey