A pathologist experiments with a deaf-mute woman who is unable to scream to prove that humans die of fright due to an organism he names The Tingler that lives within each person on the spinal cord and is suppressed only when people scream when scared.

Vincent Priceas Dr. Warren Chapin
Philip Coolidgeas Oliver 'Ollie' Higgins
Judith Evelynas Mrs. Martha Ryerson Higgins
Darryl Hickmanas David Morris
Pamela Lincolnas Lucy Stevens
Patricia Cuttsas Isabel Stevens Chapin
William Castleas Prologue Host (uncredited)
Bob Gundersonas Ryerson - Screaming Convict (uncredited)
Dal McKennonas Projectionist (uncredited)
Wilfrid M. Cline