Gru is recruited by the Anti-Villain League to help deal with a powerful new super criminal.

Steve Carellas Gru (voice)
Kristen Wiigas Lucy (voice)
Benjamin Brattas Eduardo / El Macho (voice)
Miranda Cosgroveas Margo (voice)
Dana Gaier Ellisas Edith (voice)
Elsie Fisheras Agnes (voice)
Russell Brandas Dr. Nefario (voice)
Ken Jeongas Floyd (voice)
Steve Cooganas Silas (voice)
Moisés Ariasas Antonio (voice)